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How To Calculate Rf Values Chromatography

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How To Calculate Rf Values Chromatography . Rf value = distance travelled by the spot/ distance travelled by the solvent = ⅗ = 0.6. For a given system at a known temperature, it is a characteristic of the component and can be used to identify components. Rf Value Equation Chromatography Tessshebaylo from www.tessshebaylo.com The table of protein composition may prove useful as you analyse the hydrolysates. Calculate the rf value of the red spot. A solvent which has a strong interaction for a specific analyte will more easily remove and carry that component forward in a certain period.